AJP - Lung AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (February 14, 2003). doi:10.1152/ajplung.00359.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/1/L149    most recent
00359.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abdullah, L. H.
Right arrow Articles by Davis, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abdullah, L. H.
Right arrow Articles by Davis, C. W.
Submitted on October 28, 2002
Accepted on February 10, 2003

Mucin secretion and PKC isoforms in the SPOC1 airway goblet cell line: differential effects of activation by purinergic agonist and PMA

Lubna H. Abdullah1, Jason T. Bundy1, Camille Ehre1, and C. William Davis2*

1 Cystic Fibrosis/Pulmonary Research & Treatment Center, University of North Carolina, Chapel Hill, NC, USA
2 Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, NC, USA; Cystic Fibrosis/Pulmonary Research & Treatment Center, University of North Carolina, Chapel Hill, NC, USA

* To whom correspondence should be addressed. E-mail: cwdavis{at}med.unc.edu.

SPOC1 cells, a rat trachea-derived, mucin-secreting model of airway goblet cells, possess a luminal P2Y2 purinoceptor through which UTP, ATP, and ATP{gamma}S stimulate secretion with EC50s of about 3 µM. PMA elicits mucin secretion with high EC50 (75 nM) and saturation (300 nM) values. For the first time in an airway mucin-secreting cell model, the PKC isoforms expressed and those activated by secretagogue have been determined. By RT-PCR/restriction enzyme mapping and Western blotting with isoform-specific antibodies, five isoforms were expressed in SPOC1 cells: cPKC{alpha}, nPKC{delta}, nPKC{eta}, aPKC{zeta}, and aPKC{iota}/{lambda}. PMA caused cPKC{alpha} and nPKC{delta}, only, to translocate to the membrane fraction of SPOC1 cells, and only nPKC{delta} so responded to ATP{gamma}S. In concentration:effect studies with ATP{gamma}S, membrane-associated nPKC{delta} and mucin secretion increased in parallel, with EC50s of 2 - 3 µM and maximums of 100 µM. PMA effects to increase membrane-associated cPKC{alpha} and nPKC{delta} in similar studies saturated at 30 nM, whereas mucin secretion saturated at 300 nM, suggesting a significant PKC-independent effect of PMA on mucin secretion. A prime candidate for an alternate phorbol ester receptor in SPOC1 cells is the C1 domain protein, MUNC13. RT-PCR revealed the expression of ubMUNC13-2, and its putative binding protein, DOC2{gamma}. Hence, P2Y2 (= P2U) agonists selectively activate nPKC{delta} in SPOC1 cells. PMA activates cPKC{alpha} and nPKC{delta} at high affinity, and also stimulates a lower affinity, PKC-independent pathway leading to mucin granule exocytosis.




This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
F. Chappe, M. E. Loewen, J. W. Hanrahan, and V. Chappe
Vasoactive Intestinal Peptide Increases Cystic Fibrosis Transmembrane Conductance Regulator Levels in the Apical Membrane of Calu-3 Cells through a Protein Kinase C-Dependent Mechanism
J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 226 - 238.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. Zhu, C. Ehre, L. H. Abdullah, J. K. Sheehan, M. Roy, C. M. Evans, B. F. Dickey, and C. W. Davis
Munc13-2-/- baseline secretion defect reveals source of oligomeric mucins in mouse airways
J. Physiol., April 1, 2008; 586(7): 1977 - 1992.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J.-A. Park, A. L. Crews, W. R. Lampe, S. Fang, J. Park, and K. B. Adler
Protein Kinase C{delta} Regulates Airway Mucin Secretion via Phosphorylation of MARCKS Protein
Am. J. Pathol., December 1, 2007; 171(6): 1822 - 1830.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Ehre, Y. Zhu, L. H. Abdullah, J. Olsen, K. I. Nakayama, K. Nakayama, R. O. Messing, and C. W. Davis
nPKC{varepsilon}, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells
Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1445 - C1454.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. H. Abdullah and C. W. Davis
Regulation of airway goblet cell mucin secretion by tyrosine phosphorylation signaling pathways
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L591 - L599.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. H. Rossi, W. C. Salmon, M. Chua, and C. W. Davis
Calcium signaling in human airway goblet cells following purinergic activation
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L92 - L98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Ehre, A. H. Rossi, L. H. Abdullah, K. De Pestel, S. Hill, J. C. Olsen, and C. W. Davis
Barrier role of actin filaments in regulated mucin secretion from airway goblet cells
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C46 - C56.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. A. Kemp, R. A. Sugar, and A. D. Jackson
Nucleotide-Mediated Mucin Secretion from Differentiated Human Bronchial Epithelial Cells
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 446 - 455.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.