AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (December 7, 2007). doi:10.1152/ajplung.00370.2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
294/2/L225    most recent
00370.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, H.-S.
Right arrow Articles by Sanchez-Esteban, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, H.-S.
Right arrow Articles by Sanchez-Esteban, J.
Submitted on September 7, 2007
Accepted on December 4, 2007

Interleukin-10 Protects Cultured Fetal Rat Type II Epithelial cells from Injury Induced by Mechanical Stretch

Hyeon-Soo Lee1, Yulian Wang1, Benjamin S Maciejewski1, Kenny Esho1, Christiaan Fulton1, Surendra Sharma1, and Juan Sanchez-Esteban1*

1 Pediatrics, Women & Infants Hospital of Rhode Island and the Warren Alpert Medical School of Brown University, Providence, Rhode Island, United States

* To whom correspondence should be addressed. E-mail: jsanchezesteban{at}wihri.org.

Mechanical ventilation plays a central role in the pathogenesis of bronchopulmonary dysplasia (BPD). However, the mechanisms by which excessive stretch of fetal or neonatal type II epithelial cells contributes to lung injury are not well defined. In these investigations, isolated E19 fetal rat type II epithelial cells were cultured on substrates coated with fibronectin and exposed to 5% or 20% cyclic stretch to simulate mechanical forces during lung development or lung injury, respectively. 20% stretch of fetal type II epithelial cells increased necrosis, apoptosis and proliferation when compared to control, unstretched samples. By ELISA and real-time PCR (qRT-PCR), 20% stretch increased secretion of IL-8 into the media and IL-8 gene expression and inhibited IL-10 release. Interestingly, administration of recombinant IL-10 prior to 20% stretch did not affect cell lysis but significantly reduced apoptosis and IL-8 release when compared to stretched samples without IL-10. Collectively, our studies suggest that IL-10 may play an important role in protection of fetal type II epithelial cells from injury secondary to stretch.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
Y. Wang, B. S. Maciejewski, D. Soto-Reyes, H.-S. Lee, D. Warburton, and J. Sanchez-Esteban
Mechanical stretch promotes fetal type II epithelial cell differentiation via shedding of HB-EGF and TGF-{alpha}
J. Physiol., April 15, 2009; 587(8): 1739 - 1753.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. R. Pedreira, E. Garcia-Prieto, D. Parra, A. Astudillo, E. Diaz, F. Taboada, and G. M. Albaiceta
Effects of melatonin in an experimental model of ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L820 - L827.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.