All forms of pulmonary hypertension are characterized by structural changes in pulmonary arteries. Increased numbers of cells expressing -SM-actin is a near universal finding in the remodeled artery. Traditionally, it was assumed that resident SMC were the exclusive source of these newly appearing -SM-actin-expressing cells. However, rapidly emerging experimental evidence suggests other, alternative cellular sources of these cells. One possibility is that endothelial cells can transition into mesenchymal cells expressing -SM-actin, and that this process contributes to the accumulation of SM-like cells in vascular pathologies. We review the evidence that endothelial-mesenchymal transition is an important contributor to cardiac and vascular development as well as to pathophysiologic vascular remodeling. Recent work has provided evidence for the role of TGF-, Wnt, and Notch signaling in this process. The potential roles of matrix metalloproteinases and serine proteases are also discussed. Importantly, endothelial-mesenchymal transition may be reversible. Thus insights into the mechanisms controlling endothelial-mesenchymal transition are relevant to vascular remodeling and are important as we consider new therapies aimed at reversing pulmonary vascular remodeling.