AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (December 14, 2001). doi:10.1152/ajplung.00379.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/L1004    most recent
00379.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyen, N. M
Right arrow Articles by Senior, R. M
Right arrow Search for Related Content
PubMed
Right arrow Articles by Nguyen, N. M
Right arrow Articles by Senior, R. M

Articles in PresS, published online ahead of print December 14, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00379.2001
Submitted on September 24, 2001
Accepted on December 1, 2001

LAMININ {alpha} CHAIN EXPRESSION AND BASEMENT MEMBRANE FORMATION BY RESPIRATORY EPITHELIAL CELLS, MLE-15

Nguyet M Nguyen1, Yushi Bai2, Katsumi Mochitate2, and Robert M Senior1*

1 Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, Missouri, USA
2 Environmental Health Sciences Division, National Institute for Environmental Studies, Tsubuka, Ibaraki, Japan

* To whom correspondence should be addressed. E-mail: seniorr{at}msnotes.wustl.edu.

Basement membranes have a critical role in alveolar structure and function. Alveolar type II cells make basement membrane constituents including laminin, but relatively little is known about the production of basement membrane proteins by murine alveolar type II cells and a convenient system is not available to study basement membrane production by murine alveolar type II cells. To facilitate study of basement membrane production, with particular focus on laminin chains, we examined transformed murine distal respiratory epithelial cells, MLE-15, which have many structural and biochemical features of alveolar type II cells. We found that MLE-15 cells produce laminin {alpha}5, a trace amount of laminin {alpha}3, laminins ß1 and {gamma}1, type IV collagen, and perlecan. TGF-ß1 significantly induces expression of laminin {alpha}1. When grown on a fibroblast-embedded collagen gel, MLE-15 cells assemble a basement membrane-like layer containing laminin {alpha}5. These findings indicate that MLE-15 cells will be useful to model basement membrane production and assembly by alveolar type II cells.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Pierce, C. H. Moore, and M. C. Arikan
Positive transcriptional regulatory element located within exon 1 of elastin gene.
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L391 - L399.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. B. Wallach-Dayan, G. Izbicki, P. Y. Cohen, R. Gerstl-Golan, A. Fine, and R. Breuer
Bleomycin initiates apoptosis of lung epithelial cells by ROS but not by Fas/FasL pathway
Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L790 - L796.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P.E. Christie, M. Jonas, C-H. Tsai, E.Y. Chi, and W.R. Henderson Jr
Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone
Eur. Respir. J., July 1, 2004; 24(1): 107 - 115.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2001 by the American Physiological Society.