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1 Pulmonary and Critical Care Medicine, Boston University School of Medicine, Boston, MA, USA
* To whom correspondence should be addressed. E-mail: dkotton{at}lung.bumc.bu.edu.
Although the function of the cell surface protein Sca-1 has not been identified, expression of this molecule is a characteristic of bone marrow-derived hematopoietic stem cell populations. Expression of Sca-1, however, is not restricted to hematopoietic tissue. By RT-PCR and Western analysis, we found that Sca-1 is expressed in the adult mouse lung. Sca-1 immunohistochemistry revealed a linear staining pattern on the endothelial surface of large and small pulmonary arteries and veins, and alveolar capillaries. Expression of Sca-1 in the pulmonary endothelium was confirmed by dual fluorescent microscopy on lung sections, and by FACS analysis of digested lung tissue; each of these methods showed co-localization with the endothelial marker, PECAM-1. In the kidney, Sca-1 expression was also noted in large vessels, but in contrast to the lung was not observed in capillaries. Overall, our data indicate that Sca-1 expression helps define the surface phenotype of endothelial cells throughout the pulmonary vasculature.
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