| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Pharmacology, University of Sydney, Sydney, NSW, Australia
* To whom correspondence should be addressed. E-mail: judblack{at}med.usyd.edu.au.
The protease activated receptor-2 (PAR-2) is present on human airway smooth muscle (ASM) cells and can be activated by mast cell tryptase, trypsin or an activating peptide (AP). Trypsin induced significant increases in PGE2 release from human ASM cells after 6 and 24 hours and also induced COX-2 mRNA expression and COX-2 protein. Tryptase and the PAR-2 AP did not alter PGE2 release or COX-2 protein levels suggesting a lack of PAR-2 involvement. When we compared results in asthmatic and non-asthmatic muscle cells, both trypsin and bradykinin induced less PGE2 from asthmatic ASM cells and bradykinin induced significantly less COX-2 mRNA in asthmatic cells. Significantly less PGE2 was released from proliferating ASM cells from asthmatic patients. In conclusion, trypsin induces PGE2 release and COX-2 in human ASM cells, which is unlikely to be via PAR-2 activation. In addition, ASM cells from asthmatic patients produce significantly less PGE2 and COX-2 compared with non-asthmatic cells. These findings may contribute to the increase in muscle mass evident in asthmatic airways.
This article has been cited by other articles:
|
|
 |
|
  N. Regamey, M. Ochs, T. N. Hilliard, C. Muhlfeld, N. Cornish, L. Fleming, S. Saglani, E. W. F. W. Alton, A. Bush, P. K. Jeffery, et al. Increased Airway Smooth Muscle Mass in Children with Asthma, Cystic Fibrosis, and Non-Cystic Fibrosis Bronchiectasis Am. J. Respir. Crit. Care Med., April 15, 2008; 177(8): 837 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bradding Mast cell regulation of airway smooth muscle function in asthma Eur. Respir. J., May 1, 2007; 29(5): 827 - 830. [Full Text] [PDF]
|
|
|
|
|
|
J. Chhabra, Y-Z. Li, H. Alkhouri, A. E. Blake, Q. Ge, C. L. Armour, and J. M. Hughes Histamine and tryptase modulate asthmatic airway smooth muscle GM-CSF and RANTES release Eur. Respir. J., May 1, 2007; 29(5): 861 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Borger, M. Tamm, J. L. Black, and M. Roth Asthma: Is It Due to an Abnormal Airway Smooth Muscle Cell? Am. J. Respir. Crit. Care Med., August 15, 2006; 174(4): 367 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gatti, E. Andre, S. Amadesi, T. Q. Dinh, A. Fischer, N. W. Bunnett, S. Harrison, P. Geppetti, and M. Trevisani Protease-activated receptor-2 activation exaggerates TRPV1-mediated cough in guinea pigs J Appl Physiol, August 1, 2006; 101(2): 506 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Sutcliffe, D Kaur, S Page, L Woodman, C L Armour, M Baraket, P Bradding, J M Hughes, and C E Brightling Mast cell migration to Th2 stimulated airway smooth muscle from asthmatics Thorax, August 1, 2006; 61(8): 657 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sharma, H. L. Goh, N. Asokananthan, A. Bakker, G. A. Stewart, and H. W. Mitchell Delayed and persistent suppression of bronchoconstriction by trypsin in the airway lumen Eur. Respir. J., January 1, 2006; 27(1): 20 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Burgess, Q. Ge, M. H. Poniris, S. Boustany, S. M. Twigg, J. L. Black, and P. R. A. Johnson Connective tissue growth factor and vascular endothelial growth factor from airway smooth muscle interact with the extracellular matrix Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L153 - L161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-L. N. Huynh, K. C. Malcolm, C. Kotaru, J. A. Tilstra, J. Y. Westcott, V. A. Fadok, and S. E. Wenzel Defective Apoptotic Cell Phagocytosis Attenuates Prostaglandin E2 and 15-Hydroxyeicosatetraenoic Acid in Severe Asthma Alveolar Macrophages Am. J. Respir. Crit. Care Med., October 15, 2005; 172(8): 972 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Black, Q. Ge, S. Boustany, P. R. A. Johnson, M. H. Poniris, A. R. Glanville, B. G. G. Oliver, L. M. Moir, and J. K. Burgess In vitro studies of lymphangioleiomyomatosis Eur. Respir. J., October 1, 2005; 26(4): 569 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Brightling, A. J. Ammit, D. Kaur, J. L. Black, A. J. Wardlaw, J. M. Hughes, and P. Bradding The CXCL10/CXCR3 Axis Mediates Human Lung Mast Cell Migration to Asthmatic Airway Smooth Muscle Am. J. Respir. Crit. Care Med., May 15, 2005; 171(10): 1103 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.G. Stewart Emigration and immigration of mesenchymal cells: a multicultural airway wall Eur. Respir. J., October 1, 2004; 24(4): 515 - 517. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
