Submitted on October 10, 2007
Accepted on March 4, 2008
Contributions of Nitric Oxide Synthase Isoforms to Pulmonary Oxygen Toxicity, Local vs. Mediated Effects
Ivan T Demchenko1, Dmitriy N. Atochin2, Diana R. Gutsaeva3, Ryan R. Godfrey4, Paul L Huang4, Claude A Piantadosi5, and Barry W Allen6*
1 Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Durham, North Carolina, United States; Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, United States; Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation
2 Cardiology Division, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, United States; Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation
3 Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Durham, North Carolina, United States; Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation
4 Cardiology Division, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, United States
5 Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Durham, North Carolina, United States; Division of Pulmonary Medicine, Department of Medicine, Duke University Medical Center, Durham, North Carolina, United States; Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, United States
6 Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Durham, North Carolina, United States; Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, United States
* To whom correspondence should be addressed. E-mail: bworth{at}duke.edu.
Reactive species of oxygen and nitrogen have been collectively implicated in pulmonary oxygen toxicity, but the contributions of specific molecules are unknown. Therefore, we assessed the roles of several reactive species, particularly nitric oxide, in pulmonary injury by exposing wild-type mice and seven groups of genetically altered mice to >98% O2 at 1, 3 or 4 atmospheres absolute. Genetically altered animals included knockouts lacking either neuronal nitric oxide synthase (nNOS-/-), endothelial nitric oxide synthase (eNOS-/-), inducible nitric oxide synthase (iNOS-/-), extracellular superoxide dismutase (SOD3-/-) or glutathione peroxidase 1 (GPx1-/-), as well as two transgenic variants (S1179A and S1179D) having altered eNOS activities. We confirmed our earlier finding that normobaric hyperoxia (NBO2) and hyperbaric hyperoxia (HBO2) result in at least two distinct but overlapping patterns of pulmonary injury. Our new findings are that the role of nitric oxide in the pulmonary pathophysiology of hyperoxia depends both on the specific NOS isozyme that is its source and on the level of hyperoxia. Thus, iNOS predominates in the etiology of lung injury in NBO2, and SOD3 provides an important defense. But in HBO2, nNOS is a major contributor to pulmonary injury, while eNOS is protective. In addition, we demonstrated that nitric oxide derived from nNOS is involved in a neurogenic mechanism of HBO2-induced lung injury that is linked to CNS oxygen toxicity through adrenergic/cholinergic pathways.
