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Am J Physiol Lung Cell Mol Physiol (February 1, 2002). doi:10.1152/ajplung.00447.2001
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Articles in PresS, published online ahead of print February 1, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00447.2001
Submitted on November 20, 2001
Accepted on January 27, 2002

Composition, biophysical properties and morphometry of plasmamembranes in pulmonary interstitial edema

Paola Palestini1, Chiara Calvi1, Elena Conforti1, Laura Botto1, Carla Fenoglio2, and Giuseppe Miserocchi1*

1 Experimental, Environmental Medicine and Biotechnology, Universita Milano-Bicocca, Monza, Milano, Italy
2 Animal Biology, Universita di Pavia, Pavia, Pavia, Italy

* To whom correspondence should be addressed. E-mail: giuseppe.miserocchi{at}unimib.it.

We evaluated the changes in plasmamembrane composition, biophysical properties and morphology of pulmonary endothelial cells in anesthetized rabbits receiving 0.5 ml/(kg*min) saline infusion for 180 min causing mild interstitial edema. Plasmamembrane fractions were obtained from lung homogenates with gradient centrifugation, allowing a six fold enrichment in caveolin-1. In edematous lungs, cholesterol content and phospholipidic phosphorus increased by 15% and 40%, respectively. These data correlated with morphometric analysis of lungs fixed in situ by vascular perfusion with 2.5% glutaraldehyde, suggesting a relative increase in surface of luminal to interstitial front of the capillary endothelial cells, due to a convoluted luminal profile. In edematous lungs the fraction of double bound fatty acids increased in membrane lipids; moreover, the phosphatidylcholine/phosphatidylethanolamine and the cholesterol/phospholipid ratios decreased. These changes were consistent with the increase in fluorescence anisotropy of plasmamembrane indicating an increase in its fluidity. Data suggest that mechanical stimuli elicited by a modest (~ 4 %) increase in extravascular water cause marked changes in plasmamembranes that may be of relevance in signal transduction and endothelial cell activation.




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