| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print May 3, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00478.2001
Submitted on December 14, 2001
Accepted on April 30, 2002
1 Department of Anesthesiology, Erasmus University Rotterdam, Rotterdam, The Netherlands
2 Laboratory of Pediatrics, Erasmus University Rotterdam, Rotterdam, The Netherlands
* To whom correspondence should be addressed. E-mail: vaniwaarden{at}kgk.fgg.eur.nl.
The uptake of fluorescent-labeled liposomes (with a surfactant-like composition) by alveolar macrophages and alveolar type II cells was studied using flow cytometry: in vivo by instillation of the labeled liposomes in the trachea of ventilated rats followed by isolation of the alveolar cells and determination of the cell-associated fluorescence and in vitro by incubation of isolated alveolar cells with the fluorescent liposomes. The results show that the uptake of liposomes by the alveolar cells is time- and concentration dependent. In vivo alveolar macrophages internalize more than three times as many liposomes as alveolar type II cells, whereas in vitro the amount of internalized liposomes by these cells is approximately the same. vitro practically all the cells (70 to 75%) internalize liposomes, whereas in vivo only 30% of the alveolar type II cells ingest liposomes versus 70% of the alveolar macrophages. These results indicate that in vivo only a small subpopulation of alveolar type II cells is able to internalize surfactant liposomes.
This article has been cited by other articles:
|
|
 |
|
  A. Forbes, M. Pickell, M. Foroughian, L.-J. Yao, J. Lewis, and R. Veldhuizen Alveolar macrophage depletion is associated with increased surfactant pool sizes in adult rats J Appl Physiol, August 1, 2007; 103(2): 637 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gille, B. Spring, W. Bernhard, C. Gebhard, D. Basile, K. Lauber, C. F. Poets, and T. W. Orlikowsky Differential effect of surfactant and its saturated phosphatidylcholines on human blood macrophages J. Lipid Res., February 1, 2007; 48(2): 307 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Frank, C. M. Wray, D. F. McAuley, R. Schwendener, and M. A. Matthay Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injury Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1191 - L1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jain, C. Dodia, A. B. Fisher, and S. R. Bates Pathways for clearance of surfactant protein A from the lung Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1011 - L1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. H. Poelma, B. Lachmann, J. J. Haitsma, L. J. Zimmermann, and J. F. van Iwaarden Influence of phosphatidylglycerol on the uptake of liposomes by alveolar cells and on lung function J Appl Physiol, May 1, 2005; 98(5): 1784 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. H. Poelma, L. J. Zimmermann, W. A. van Cappellen, J. J. Haitsma, B. Lachmann, and J. F. van Iwaarden Distinct effects of SP-B and SP-C on the uptake of surfactant-like liposomes by alveolar cells in vivo and in vitro Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L1056 - L1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Poelma, M. R. Ju, S. C. Bakker, L. J. Zimmermann, B. F. Lachmann, and J. F. van Iwaarden A Common Pathway for the Uptake of Surfactant Lipids by Alveolar Cells Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 751 - 758. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
