AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (April 28, 2006). doi:10.1152/ajplung.00484.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
291/3/L417    most recent
00484.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyen, N.-B.
Right arrow Articles by Yang, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyen, N.-B.
Right arrow Articles by Yang, F.
Submitted on November 14, 2005
Accepted on April 27, 2006

Hepcidin expression and iron transport in alveolar macrophages

Ngoc-Bich Nguyen1, Kimberly D Callaghan1, Andrew J Ghio2, David J Haile3, and Funmei Yang1*

1 Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States
2 Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States; Human Studies Division, US Environmental Protection Agency, Chapel Hill, North Carolina, United States
3 Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States; Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States

* To whom correspondence should be addressed. E-mail: yangf{at}uthscsa.edu.

Alveolar macrophages express many proteins important in iron homeostasis including the iron importer DMT1 and the iron exporter FPN1 that likely participate in lung defense. We found the iron regulatory hormone hepcidin (HAMP) is also produced by alveolar macrophages. In mouse alveolar macrophages, HAMP mRNA was detected at a low level when not stimulated but at a high level when exposed to lipopolysaccharide (LPS). LPS also affected the mRNA levels of the iron transporters with DMT1 being up-regulated and FPN1 down-regulated. However, iron had no effect on HAMP expression but was able to up-regulate both DMT1 and FPN1 in alveolar macrophages. IL-1 and IL-6, which are important in HAMP augmentation in hepatocytes, also did not affect HAMP expression in alveolar macrophages. In fact, the LPS-induced alterations in the expression of HAMP as well as DMT1 and FPN1 were preserved in the alveolar macrophages isolated from IL-1 receptor or IL-6 deficient mice. When alveolar macrophages were loaded with transferrin-bound 55Fe, the subsequent release of 55Fe was significantly inhibited by LPS. In addtion, treatment of these cells with either LPS or HAMP caused the diminishment of the surface FPN1. These findings are consistent with the current model that HAMP production leads to a decreased iron efflux. Our studies suggests that iron mobilization by alveolar macrophages can be affected by iron and LPS via several pathways including HAMP-mediated degradation of FPN1, and that these cells may use unique regulatory mechanisms to cope with iron imbalance in the lung.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
F. B. Sow, G. R. Alvarez, R. P. Gross, A. R. Satoskar, L. S. Schlesinger, B. S. Zwilling, and W. P. Lafuse
Role of STAT1, NF-{kappa}B, and C/EBP{beta} in the macrophage transcriptional regulation of hepcidin by mycobacterial infection and IFN-{gamma}
J. Leukoc. Biol., November 1, 2009; 86(5): 1247 - 1258.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
P. Matak, T. B. Chaston, B. Chung, S. K. Srai, A. T. McKie, and P. A. Sharp
Activated macrophages induce hepcidin expression in HuH7 hepatoma cells
Haematologica, June 1, 2009; 94(6): 773 - 780.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. F. Collins, M. Wessling-Resnick, and M. D. Knutson
Hepcidin Regulation of Iron Transport
J. Nutr., November 1, 2008; 138(11): 2284 - 2288.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. Aigner, I. Theurl, M. Theurl, D. Lederer, H. Haufe, O. Dietze, M. Strasser, C. Datz, and G. Weiss
Pathways underlying iron accumulation in human nonalcoholic fatty liver disease
Am. J. Clinical Nutrition, May 1, 2008; 87(5): 1374 - 1383.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Theurl, M. Theurl, M. Seifert, S. Mair, M. Nairz, H. Rumpold, H. Zoller, R. Bellmann-Weiler, H. Niederegger, H. Talasz, et al.
Autocrine formation of hepcidin induces iron retention in human monocytes
Blood, February 15, 2008; 111(4): 2392 - 2399.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
F. B. Sow, W. C. Florence, A. R. Satoskar, L. S. Schlesinger, B. S. Zwilling, and W. P. Lafuse
Expression and localization of hepcidin in macrophages: a role in host defense against tuberculosis
J. Leukoc. Biol., October 1, 2007; 82(4): 934 - 945.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
J. l. Sullivan
Macrophage Iron, Hepcidin, and Atherosclerotic Plaque Stability
Experimental Biology and Medicine, September 1, 2007; 232(8): 1014 - 1020.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.