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Am J Physiol Lung Cell Mol Physiol (March 29, 2002). doi:10.1152/ajplung.00492.2001
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Articles in PresS, published online ahead of print March 29, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00492.2001
Submitted on December 26, 2001
Accepted on March 26, 2002

Molecular Cloning of Rat Actin Filament Associated Protein: a Putative Adaptor in Mechanical Stretch-Induced c-Src Activation

Monika Lodyga1, Xiao-Hui Bai1, Eric Mourgeon1, Bing Han1, Shaf Keshavjee2, and Mingyao Liu2*

1 Division of Cellular and Molecular Biology, University Health Network Toronto General Hospital Research Institute, Toronto, Ontario, Canada
2 Division of Cellular and Molecular Biology, University Health Network Toronto General Hospital Research Institute, Toronto, Ontario, Canada; Surgery, University of Tennessee, Toronto, Ontario, Canada

* To whom correspondence should be addressed. E-mail: mingyao.liu{at}utoronto.ca.

Mechanical stretch-induced activation of c-Src is an important step for signal transduction of stretch-induced fetal rat lung cell proliferation. This process appears to be mediated through actin filament associated protein (AFAP), encoded by a gene originally cloned from chicken. In the present study, we cloned rat AFAP gene from fetal rat lungs. Its mRNA and protein are differentially expressed among various tissues. The protein is co-localized with actin filaments in fetal rat lung epithelial cells and fibroblasts. Mechanical stretch increased tyrosine phosphorylation of rat AFAP and its binding to c-Src within the initial several minutes. Src SH2 and SH3 binding motifs are highly conserved in the AFAP proteins (from chicken, rat to human). Based on the molecular structure of AFAP protein, we speculate that it is an adaptor in mechanical stretch-induced activation of c-Src. A novel model of mechanoreception is proposed.




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