AJP - Lung AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (April 26, 2002). doi:10.1152/ajplung.00496.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/L636    most recent
00496.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, J. B
Right arrow Articles by Rovai, L. E
Right arrow Search for Related Content
PubMed
Right arrow Articles by Smith, J. B
Right arrow Articles by Rovai, L. E

Articles in PresS, published online ahead of print April 26, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00496.2001
Submitted on January 4, 2002
Accepted on April 11, 2002

Glucocorticoid-attenuated response genes induced in the lung during endotoxemia

Jeffrey B Smith1*, Tam T Nguyen1, Heather J Hughes1, Harvey R Herschman2, Daniel P Widney1, Kim C Bui1, and Leonor E Rovai1

1 Department of Pediatrics, UCLA School of Medicine, Los Angeles, CA, USA
2 Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, CA, USA; Department of Molecular Pharmacology, UCLA School of Medicine, Los Angeles, CA, USA; Department of Molecular Biology Institute, UCLA, Los Angeles, CA, USA

* To whom correspondence should be addressed. E-mail: jbsmith{at}ucla.edu.

Cytokines and other mediators whose induction in inflammatory lung disease is attenuated by glucocorticoids are potential targets for development of selective anti-inflammatory treatments. We refer to genes with these regulatory characteristics as Glucocorticoid-Attenuated Response Genes, or GARGs. Systematic identification of GARGs has not been attempted previously in vivo. Using an endotoxemia model in adrenalectomized mice, we constructed a subtracted lung library enriched in endotoxemia-induced genes, and identified candidate GARGs by differential hybridization screening. Northern analysis confirmed induction in the lung during endotoxemia and attenuation by glucocorticoids of 36 genes of diverse types. The majority were genes of unknown function not previously implicated in the pulmonary response to inflammation, including a new member of a 2'-5' oligoadenylate synthetase-like family (murine OASL-2), and a novel lung-inducible Neuralized-related C3HC4 RING protein (LINCR). Our results suggest that a full understanding of glucocorticoid effects on lung inflammation will require elucidation of the roles of an extensive network of glucocorticoid-modulated genes.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C. Commisso and G. L. Boulianne
The NHR1 Domain of Neuralized Binds Delta and Mediates Delta Trafficking and Notch Signaling
Mol. Biol. Cell, January 1, 2007; 18(1): 1 - 13.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. B. Smith and H. R. Herschman
Targeted Identification of Glucocorticoid-attenuated Response Genes: In Vitro and in Vivo Models
Proceedings of the ATS, November 1, 2004; 1(3): 275 - 281.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.