| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Lovelace Respiratory Research Institute, Alburqueque, New Mexico, United States
2 Asthma and Immunology, Lovelace Respiratory Research Institute, Albuquerque, New Mexico, United States
3 Lovelace Respiratory Research Institute, Alburquerque, New Mexico, United States
4 The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
5 Lovelace Respiratory Research Institute, Albuquerque, New Mexico, United States; The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
* To whom correspondence should be addressed. E-mail: ytesfaig{at}lrri.org.
Immunological tolerance during prolonged exposure to allergen is accompanied by a shift in the lymphocyte content and a reduction of goblet cell metaplasia (GCM). Bim initiates negative selection of autoreactive T and B cells and shut-down of T cell immune responses in vivo. The present study investigated whether Bim plays a role in the resolution of GCM during prolonged exposure to allergen. Loss of Bim increased T lymphocyte numbers in the bronchoalveolar lavage at 4 and 15 d of allergen exposure. The numbers of pulmonary CD4+8-, CD4-8+, and 
T cells were significantly higher in naive and allergen-challenged bim-/- mice compared to wt littermates. When activated, pulmonary bim-/- T cells produced increased levels of IFN compared to bim+/+ T cells. No differences were noted in the total numbers of epithelial cells per mm of basal lamina between bim+/+ and bim-/- mice and the rate of resolution over 15-d exposure was similar in both groups of mice. However, GCM was significantly enhanced and expression of IL-13R
2 was reduced in bim-/- mice compared to wt mice at 4 d. Furthermore, treatment of bronchiolar explant cultures with increasing IFN levels reduced immunostaining for IL-13R2. Collectively, these studies suggest that during prolonged exposure to allergen, Bim plays no role in the resolution of GCM but increased IFN levels in bim-/- mice may be responsible for reduced expression of IL-13R2 and enhanced GCM despite similar levels of IL-13 in bim+/+ and bim-/- mice.
This article has been cited by other articles:
|
|
 |
|
  Y. A. Mebratu, B. F. Dickey, C. Evans, and Y. Tesfaigzi The BH3-only protein Bik/Blk/Nbk inhibits nuclear translocation of activated ERK1/2 to mediate IFN{gamma}-induced cell death J. Cell Biol., November 3, 2008; 183(3): 429 - 439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schwalm, J. F. Stevens, Z. Jiang, M. R. Schuyler, R. Schrader, S. H. Randell, F. H. Y. Green, and Y. Tesfaigzi Expression of the proapoptotic protein Bax is reduced in bronchial mucous cells of asthmatic subjects Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1102 - L1109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xiang, J. Rir-Sim-Ah, and Y. Tesfaigzi IL-9 and IL-13 Induce Mucous Cell Metaplasia That Is Reduced by IFN-{gamma} in a Bax-Mediated Pathway Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 310 - 317. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
