AJP - Lung AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L650-L658, 2002. First published December 7, 2001; doi:10.1152/ajplung.00370.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/4/L650    most recent
00370.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lazrak, A.
Right arrow Articles by Matalon, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lazrak, A.
Right arrow Articles by Matalon, S.
Vol. 282, Issue 4, L650-L658, April 2002

SPECIAL TOPIC
Alveolar Epithelial Ion and Fluid Transport
cAMP regulation of Clminus and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> secretion across rat fetal distal lung epithelial cells

Ahmed Lazrak1, Ulrich Thome2, Carpantanto Myles1, Janice Ware2, Lan Chen1, Charles J. Venglarik3, and Sadis Matalon1,2,3,4

Departments of 1 Anesthesiology, 2 Pediatrics, 3 Environmental Health Sciences, and 4 Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35233

We isolated and cultured fetal distal lung epithelial (FDLE) cells from 17- to 19-day rat fetuses and assayed for anion secretion in Ussing chambers. With symmetrical Ringer solutions, basal short-circuit currents (Isc) and transepithelial resistances were 7.9 ± 0.5 µA/cm2 and 1,018 ± 73 Omega  · cm2, respectively (means ± SE; n = 12). Apical amiloride (10 µM) inhibited basal Isc by ~50%. Subsequent addition of forskolin (10 µM) increased Isc from 3.9 ± 0.63 µA/cm2 to 7.51 ± 0.2 µA/cm2 (n = 12). Basolateral bumetanide (100 µM) decreased forskolin-stimulated Isc from 7.51 ± 0.2 µA/cm2 to 5.62 ± 0.53, whereas basolateral 4,4'-dinitrostilbene-2,2'-disulfonate (5 mM), an inhibitor of HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> secretion, blocked the remaining Isc. Forskolin addition evoked currents of similar fractional magnitudes in symmetrical Cl-- or HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>-free solutions; however, no response was seen using HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>- and Cl--free solutions. The forskolin-stimulated Isc was inhibited by glibenclamide but not apical DIDS. Glibenclamide also blocked forskolin-induced Isc across monolayers having nystatin-permeablized basolateral membranes. Immunolocalization studies were consistent with the expression of cystic fibrosis transmembrane conductance regulator (CFTR) protein in FDLE cells. In aggregate, these findings indicate the presence of cAMP-activated Cl- and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> secretion across rat FDLE cells mediated via CFTR.

short-circuit current; amiloride; nystatin; swelling-activated conductance; immunocytochemistry; adenosine 3',5'-cyclic monophosphate


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. Chen, W. Song, I. C. Davis, K. Shrestha, E. Schwiebert, W. M. Sullender, and S. Matalon
Inhibition of Na+ Transport in Lung Epithelial Cells by Respiratory Syncytial Virus Infection
Am. J. Respir. Cell Mol. Biol., May 1, 2009; 40(5): 588 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. Chen, C. A. Bosworth, T. Pico, J. F. Collawn, K. Varga, Z. Gao, J. P. Clancy, J. A. Fortenberry, J. R. Lancaster Jr., and S. Matalon
DETANO and Nitrated Lipids Increase Chloride Secretion across Lung Airway Cells
Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 150 - 162.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. G. Folkesson and M. A. Matthay
Alveolar Epithelial Ion and Fluid Transport: Recent Progress
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 10 - 19.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Fang, Y. Song, J. Hirsch, L. J. V. Galietta, N. Pedemonte, R. L. Zemans, G. Dolganov, A. S. Verkman, and M. A. Matthay
Contribution of CFTR to apical-basolateral fluid transport in cultured human alveolar epithelial type II cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L242 - L249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Swystun, L. Chen, P. Factor, B. Siroky, P. D. Bell, and S. Matalon
Apical trypsin increases ion transport and resistance by a phospholipase C-dependent rise of Ca2+
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L820 - L830.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Brochiero, A. Dagenais, A. Prive, Y. Berthiaume, and R. Grygorczyk
Evidence of a functional CFTR Cl- channel in adult alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L382 - L392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Leroy, A. Dagenais, Y. Berthiaume, and E. Brochiero
Molecular identity and function in transepithelial transport of KATP channels in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1027 - L1037.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. P. Mason, M. Petersen, C. Melot, B. Imanow, O. Matveykine, M.-T. Gautier, A. Sarybaev, A. Aldashev, M. M. Mirrakhimov, B. H. Brown, et al.
Serial changes in nasal potential difference and lung electrical impedance tomography at high altitude
J Appl Physiol, May 1, 2003; 94(5): 2043 - 2050.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. O'Grady and S. Y. Lee
Chloride and potassium channel function in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L689 - L700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
U. H. Thome, I. C. Davis, S. V. Nguyen, B. J. Shelton, and S. Matalon
Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L376 - L385.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. A. Matthay
Editorial: Alveolar Epithelial Ion and Fluid Transport: Regulation of ion and fluid transport across the distal pulmonary epithelia: new insights
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L595 - L598.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online