AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L1066-L1074, 2002. First published December 21, 2001; doi:10.1152/ajplung.00195.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/L1066    most recent
00195.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Justice, J. P.
Right arrow Articles by Lee, N. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Justice, J. P.
Right arrow Articles by Lee, N. A.
Vol. 282, Issue 5, L1066-L1074, May 2002

CD4+ T cell-dependent airway mucus production occurs in response to IL-5 expression in lung

J. Paul Justice1, J. Crosby1, M. T. Borchers2, A. Tomkinson3, J. J. Lee2, and N. A. Lee1

1 Divisions of Hematology/Oncology and 2 Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259; and 3 Pharmaceutical Division, Department of Biotechnology, Bayer Corporation, Berkeley, California 94701-1986

The potential role of airway interleukin-5 (IL-5) expression in eliciting mucus production was demonstrated in a pulmonary IL-5 transgenic mouse model (NJ.1726) in which naive transgenic mice display comparable levels of airway mucus relative to allergen-sensitized and -challenged wild-type mice. The intrinsic mucus accumulation of NJ.1726 was abolished in compound transgenic-gene knockout mice deficient of either CD4+ cells [NJ.1726/CD4(-/-)] or alpha beta T cell receptor-positive (TCR+) cells [NJ.1726/alpha beta TCR(-/-)]. In addition, mucus production in naive NJ.1726 was inhibited by >90% after administration of the soluble anti-IL-4 receptor alpha -subunit antagonist. The loss of mucus production in NJ.1726/CD4(-/-), NJ.1726/alpha beta TCR(-/-), and anti-IL-4 receptor alpha -subunit antagonist-treated mice occurred notwithstanding the significant pulmonary eosinophilia and expansion of airway B cells induced by ectopic IL-5 expression. Furthermore, the loss of mucus accumulation occurred in these mice despite elevated levels of airway and peripheral IL-5, indicating that IL-5 does not directly induce goblet cell metaplasia and mucus production. Thus pulmonary expression of IL-5 alone is capable of inducing CD4+ T cell-dependent goblet cell metaplasia, apparently mediated by IL-4 receptor alpha -subunit-ligand interactions, and represents a previously unrecognized novel pathway for augmenting allergen-induced mucus production.

asthma; goblet cell; transgenic; gene knockout


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
Z. Bao, S. Guan, C. Cheng, S. Wu, S. H. Wong, D. M. Kemeny, B. P. Leung, and W. S. F. Wong
A Novel Antiinflammatory Role for Andrographolide in Asthma via Inhibition of the Nuclear Factor-{kappa}B Pathway
Am. J. Respir. Crit. Care Med., April 15, 2009; 179(8): 657 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Z. Bao, S. Lim, W. Liao, Y. Lin, C. Thiemermann, B. P. Leung, and W. S. F. Wong
Glycogen Synthase Kinase-3beta Inhibition Attenuates Asthma in Mice
Am. J. Respir. Crit. Care Med., September 1, 2007; 176(5): 431 - 438.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
J. P. Buchweitz, J. R. Harkema, and N. E. Kaminski
Time-Dependent Airway Epithelial and Inflammatory Cell Responses Induced by Influenza Virus A/PR/8/34 in C57BL/6 Mice
Toxicol Pathol, April 1, 2007; 35(3): 424 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. L. S. George, M. L. White, K. Kulhankova, A. Mahajan, P. S. Thorne, J. M. Snyder, and J. N. Kline
Early exposure to a nonhygienic environment alters pulmonary immunity and allergic responses
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L512 - L522.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. Duan, J. H. P. Chan, K. McKay, J. R. Crosby, H. H. Choo, B. P. Leung, J. G. Karras, and W. S. F. Wong
Inhaled p38{alpha} Mitogen-activated Protein Kinase Antisense Oligonucleotide Attenuates Asthma in Mice
Am. J. Respir. Crit. Care Med., March 15, 2005; 171(6): 571 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Voynow, B. M. Fischer, D. E. Malarkey, L. H. Burch, T. Wong, M. Longphre, S. B. Ho, and W. M. Foster
Neutrophil elastase induces mucus cell metaplasia in mouse lung
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1293 - L1302.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Duan, J. H. P. Chan, C. H. Wong, B. P. Leung, and W. S. F. Wong
Anti-Inflammatory Effects of Mitogen-Activated Protein Kinase Kinase Inhibitor U0126 in an Asthma Mouse Model
J. Immunol., June 1, 2004; 172(11): 7053 - 7059.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. H. Shen, S. I. Ochkur, M. P. McGarry, J. R. Crosby, E. M. Hines, M. T. Borchers, H. Wang, T. L. Biechelle, K. R. O'Neill, T. L. Ansay, et al.
A Causative Relationship Exists Between Eosinophils and the Development of Allergic Pulmonary Pathologies in the Mouse
J. Immunol., March 15, 2003; 170(6): 3296 - 3305.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Perez-Vilar, J. K. Sheehan, and S. H. Randell
Making More MUCS
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 267 - 270.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online