AJP - Lung AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L648-L654, 2002. First published May 3, 2002; doi:10.1152/ajplung.00478.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/L648    most recent
00478.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poelma, D. L. H.
Right arrow Articles by van Iwaarden, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poelma, D. L. H.
Right arrow Articles by van Iwaarden, J. F.
Vol. 283, Issue 3, L648-L654, September 2002

In vivo and in vitro uptake of surfactant lipids by alveolar type II cells and macrophages

D. L. H. Poelma1, L. J. I. Zimmermann2, H. H. Scholten1, B. Lachmann1, and J. F. van Iwaarden2

1 Department of Anesthesiology and 2 Laboratory of Pediatrics, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands

The uptake of fluorescent-labeled liposomes (with a surfactant-like composition) by alveolar macrophages and alveolar type II cells was studied using flow cytometry, in vivo by instillation of the labeled liposomes in the trachea of ventilated rats followed by isolation of the alveolar cells and determination of the cell-associated fluorescence, and in vitro by incubation of isolated alveolar cells with the fluorescent liposomes. The results show that the uptake of liposomes by the alveolar cells is time and concentration dependent. In vivo alveolar macrophages internalize more than three times as many liposomes as alveolar type II cells, whereas in vitro, the amount of internalized liposomes by these cells is approximately the same. In vitro, practically all the cells (70-75%) internalize liposomes, whereas in vivo only 30% of the alveolar type II cells ingest liposomes vs. 70% of the alveolar macrophages. These results indicate that in vivo, only a small subpopulation of alveolar type II cells is able to internalize surfactant liposomes.

pneumocyte; lung; liposome; fluorescence


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. Forbes, M. Pickell, M. Foroughian, L.-J. Yao, J. Lewis, and R. Veldhuizen
Alveolar macrophage depletion is associated with increased surfactant pool sizes in adult rats
J Appl Physiol, August 1, 2007; 103(2): 637 - 645.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. Gille, B. Spring, W. Bernhard, C. Gebhard, D. Basile, K. Lauber, C. F. Poets, and T. W. Orlikowsky
Differential effect of surfactant and its saturated phosphatidylcholines on human blood macrophages
J. Lipid Res., February 1, 2007; 48(2): 307 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Frank, C. M. Wray, D. F. McAuley, R. Schwendener, and M. A. Matthay
Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injury
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1191 - L1198.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Jain, C. Dodia, A. B. Fisher, and S. R. Bates
Pathways for clearance of surfactant protein A from the lung
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1011 - L1018.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. L. H. Poelma, B. Lachmann, J. J. Haitsma, L. J. Zimmermann, and J. F. van Iwaarden
Influence of phosphatidylglycerol on the uptake of liposomes by alveolar cells and on lung function
J Appl Physiol, May 1, 2005; 98(5): 1784 - 1791.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. L. H. Poelma, L. J. Zimmermann, W. A. van Cappellen, J. J. Haitsma, B. Lachmann, and J. F. van Iwaarden
Distinct effects of SP-B and SP-C on the uptake of surfactant-like liposomes by alveolar cells in vivo and in vitro
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L1056 - L1065.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. L. Poelma, M. R. Ju, S. C. Bakker, L. J. Zimmermann, B. F. Lachmann, and J. F. van Iwaarden
A Common Pathway for the Uptake of Surfactant Lipids by Alveolar Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 751 - 758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online