HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/L55    most recent 00198.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by von der Hardt, K. Articles by Rascher, W. Search for Related Content PubMed PubMed Citation Articles by von der Hardt, K. Articles by Rascher, W.

Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon

¹Klinik für Kinder und Jugendliche, Universität Erlangen-Nürnberg, D-91054 Erlangen; and ²Institut für Pathologie, Universität Gießen, D-35392 Gießen, Germany

Submitted 19 June 2002 ; accepted in final form 10 February 2003

The aim of this study was to identify cell types involved in the anti-inflammatory effect of ventilation with perfluorocarbon in vivo. Fifteen anesthetized, surfactant-depleted piglets received either aerosolized perfluorocarbon (Aerosol-PFC), partial liquid ventilation (rLV) at functional residual capacity (FRC) volume (FRC-PLV), or intermittent mandatory ventilation (control). After laser-assisted microdissection of different lung cell types, mRNA expression of IL-8 and ICAM-1 was determined using TaqMan real-time PCR normalized to hypoxanthine phosphoribosyltransferase (HPRT). IL-8 mRNA expression (means ± SE; control vs. Aerosol-PFC) was 356 ± 142 copies IL-8 mRNA/copy HPRT mRNA vs. 3.5 ± 1.8 in alveolar macrophages (P <0.01); 208 ± 108 vs. 2.7 ± 0.8 in bronchiolar epithelial cells (P <0.05); 26 ± 11 vs. 0.7 ± 0.2 in alveolar septum cells (P <0.01); 2.8 ± 1.0 vs. 0.8 ± 0.4 in bronchiolar smooth muscle cells (P <0.05); and 1.1 ± 0.4 vs. 0.2 ± 0.05 in vascular smooth muscle cells (P <0.05). With FRC-PLV, IL-8/HPRT mRNA expression was significantly lower in macrophages, bronchiolar epithelial, and vascular smooth muscle cells. ICAM-1 mRNA expression in vascular endothelial cells remained unchanged. Predominantly, alveolar macrophages and bronchiolar epithelial cells were involved in the inflammatory pulmonary process. The anti-inflammatory effect of Aerosol-PFC was most pronounced.

aerosolized perfluorocarbon; interleukin-8; surfactant-depleted piglets; polymerase chain reaction

This article has been cited by other articles:

				D. Haufe, E. Koenigshausen, L. Knels, M. Wendel, S. N. Stehr, and T. Koch Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L134 - L142. [Abstract] [Full Text] [PDF]

				C. E Minge, N. K. Ryan, K. H. V. D. Hoek, R. L. Robker, and R. J. Norman Troglitazone Regulates Peroxisome Proliferator-Activated Receptors and Inducible Nitric Oxide Synthase in Murine Ovarian Macrophages Biol Reprod, January 1, 2006; 74(1): 153 - 160. [Abstract] [Full Text] [PDF]

				D. Haufe, T. Luther, M. Kotzsch, L. Knels, and T. Koch Perfluorocarbon attenuates response of concanavalin A-stimulated mononuclear blood cells without altering ligand-receptor interaction Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L210 - L216. [Abstract] [Full Text] [PDF]

				M. A. Kandler, K. von der Hardt, S. Mahfoud, M. Chada, E. Schoof, T. Papadopoulos, W. Rascher, and J. Dotsch Pilot Intervention: Aerosolized Adrenomedullin Reduces Pulmonary Hypertension J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 1021 - 1026. [Abstract] [Full Text] [PDF]