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Mechanical strain increases cell stiffness through cytoskeletal filament reorganization

¹Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106; ²School of Biomedical Engineering, Dalhousie University, Halifax, Nova Scotia B3H 3J5, Canada; and ³Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115

Submitted 30 September 2002 ; accepted in final form 17 April 2003

We tested the hypothesis that cytoskeletal reorganization induced by cyclic strain increases cytoskeletal stiffness (G'). G' was measured by optical magnetic twisting cytometry in control cells and cells that had received mechanical strain for 10–12 days. G' was measured before and after both contractile and relaxant agonists, and in the strained cells both parallel (Para) and perpendicular (Perp) to the aligned cytoskeleton. Before activation, G' Para was 24 ± 5% (± SE) greater compared with Perp (P < 0.05), and 35% ± 6 greater compared with control (Cont, P < 0.01). The difference between strained and control cells was enhanced by KCl, increasing G' 171 ± 7% Para compared with 125 ± 6% Perp and 129 ± 8% Cont (P < 10^-5 both cases). The decrease in G' from baseline due to relaxant agonists isoproterenol and dibutyryl cAMP was similar in all groups. Long-term oscillatory loading of airway smooth muscle (ASM) cells caused stiffness to increase and become anisotropic. These findings are consistent with the hypothesis that cytoskeletal reorganization can enhance ASM stiffness and contractility. They imply, furthermore, that oscillatory loading of ASM may contribute to airway narrowing and failure of airway dilation in asthma.

smooth muscle contraction; plasticity; optical magnetic twisting cytometry; anisotropy; airway smooth muscle cell

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