HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/5/L1087    most recent 00409.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Singer, C. A. Articles by Gerthoffer, W. T. Search for Related Content PubMed PubMed Citation Articles by Singer, C. A. Articles by Gerthoffer, W. T.

p38 MAPK and NF-B mediate COX-2 expression in human airway myocytes

¹Department of Pharmacology and ²Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557-0046

Submitted 27 November 2002 ; accepted in final form 17 July 2003

We have previously demonstrated that p38 and extracellular signal-regulated protein kinase (ERK) mitogen-activated protein kinases (MAPK) are components of proinflammatory induced cytokine expression in human airway myocytes. The experiments described here further these studies by examining p38 MAPK and NF-B regulation of cyclooxygenase-2 (COX-2) expression in response to a complex inflammatory stimulus consisting of 10 ng/ml interleukin (IL)-1, tumor necrosis factor- (TNF-), and interferon (IFN)-. COX-2 expression was induced with this stimulus in a time-dependent manner, with maximal expression seen 12-20 h after treatment. Semiquantitative RT-PCR and immunoblotting experiments demonstrate decreased COX-2 expression following treatment with the p38 MAPK inhibitor SB-203580 (25 µM) or the proteosome inhibitor MG-132 (1 µM). SB-203580 did not affect cytokine-stimulated IB degradation, NF-B nuclear binding activity, or NF-B-dependent signaling from the COX-2 promoter, indicating that p38 MAPK and NF-B may affect COX-2 expression via separate signaling pathways. SB-203580, but not MG-132, also increased the initial rate of COX-2 mRNA decay, indicating p38 MAPK, but not NF-B, participates in the regulation of COX-2 mRNA stability. These findings suggest that although p38 MAPK and NF-B signaling regulate steady-state levels of COX-2 expression, p38 MAPK additionally affects stability of COX-2 mRNA in cytokine-stimulated human airway myocytes.

inflammation; mitogen-activated protein kinase; mRNA stability; cytokines

This article has been cited by other articles:

				T. Quante, Y. C. Ng, E. E. Ramsay, S. Henness, J. C. Allen, J. Parmentier, Q. Ge, and A. J. Ammit Corticosteroids Reduce IL-6 in ASM Cells via Up-Regulation of MKP-1 Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 208 - 217. [Abstract] [Full Text] [PDF]

				C.-C. Ho, Y.-C. Ling, L. W. Chang, H.-T. Tsai, M.-H. Tsai, and P. Lin 17-Beta Estradiol and Hydroxyestradiols Interact via the NF-Kappa B Pathway to Elevate Cyclooxygenase 2 Expression and Prostaglandin E2 Secretion in Human Bronchial Epithelial Cells Toxicol. Sci., August 1, 2008; 104(2): 294 - 302. [Abstract] [Full Text] [PDF]

				O. Tliba and Y. Amrani Airway Smooth Muscle Cell as an Inflammatory Cell: Lessons Learned from Interferon Signaling Pathways Proceedings of the ATS, January 1, 2008; 5(1): 106 - 112. [Abstract] [Full Text] [PDF]

				Y. T.Y. Li, K. E. Swales, G. J. Thomas, T. D. Warner, and D. Bishop-Bailey Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell Inflammation and Migration Arterioscler. Thromb. Vasc. Biol., December 1, 2007; 27(12): 2606 - 2611. [Abstract] [Full Text] [PDF]

				H. F. Kramer and L. J. Goodyear Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle J Appl Physiol, July 1, 2007; 103(1): 388 - 395. [Abstract] [Full Text] [PDF]

				J. Kanefsky, M. Lenburg, and C.-M. Hai Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 417 - 425. [Abstract] [Full Text] [PDF]

				R. M. Pascual, E. M. Carr, M. C. Seeds, M. Guo, R. A. Panettieri Jr., S. P. Peters, and R. B. Penn Regulatory features of interleukin-1beta-mediated prostaglandin E2 synthesis in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L501 - L508. [Abstract] [Full Text] [PDF]

				M. Wahl, T. J. Eddinger, and C.-M. Hai Sinusoidal length oscillation- and receptor-mediated mRNA expression of myosin isoforms and {alpha}-SM actin in airway smooth muscle Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1697 - C1708. [Abstract] [Full Text] [PDF]

				A. K. Mandal, Z. Zhang, R. Ray, M. S. Choi, B. Chowdhury, N. Pattabiraman, and A. B. Mukherjee Uteroglobin Represses Allergen-induced Inflammatory Response by Blocking PGD2 Receptor-mediated Functions J. Exp. Med., May 17, 2004; 199(10): 1317 - 1330. [Abstract] [Full Text] [PDF]

				G. Y. Park, M. Joo, T. Pedchenko, T. S. Blackwell, and J. W. Christman Regulation of macrophage cyclooxygenase-2 gene expression by modifications of histone H3 Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L956 - L962. [Abstract] [Full Text] [PDF]