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Am J Physiol Lung Cell Mol Physiol 286: L1095-L1104, 2004. First published January 23, 2004; doi:10.1152/ajplung.00304.2003
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TRANSLATIONAL PHYSIOLOGY
Acute Lung Injury

Proteomic analysis of pulmonary edema fluid and plasma in patients with acute lung injury

Russell P. Bowler,1 Beth Duda,1 Edward D. Chan,1 Jan J. Enghild,2 Lorraine B. Ware,3 Michael A. Matthay,4 and Mark W. Duncan5

1Department of Medicine, National Jewish Medical and Research Center, Denver 80206; 5Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, Colorado 80262; 3Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232; 4Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of California, San Francisco, California 94143; and 2Department of Molecular and Structural Biology, Århus University, 8000 Århus C, Denmark

Submitted 3 September 2003 ; accepted in final form 16 January 2004

Proteomics is the large-scale analysis of protein profiles. This approach has not yet been reported in the study of acute lung injury (ALI). This study details protein profiles in plasma and pulmonary edema fluid (EF) from 16 ALI patients and plasma and bronchoalveolar lavage fluid (BALF) from 12 normal subjects. More than 300 distinct protein spots were evident in the EF and BALF of both normal subjects and ALI patients. Of these, 158 were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In the plasma and EF protein profile of ALI patients, there were multiple qualitative changes. For instance, in all normal subjects, but in only one of the ALI patients, seven distinct surfactant protein A isoforms were evident. Nearly all ALI patients also had protein spots that indicate truncation or other posttranslational modifications. Several of these novel changes could serve as new biomarkers of lung injury.

bronchoalveolar lavage; proteomics; mass fingerprinting


Address for reprint requests and other correspondence: R. P. Bowler, National Jewish Medical and Research Center, K736a, 1400 Jackson St., Denver, CO 80206 (E-mail: BowlerR{at}njc.org).




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