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CpG DNA-mediated immune response in pulmonary endothelial cells

¹Center for Pharmacogenetics and Department of Pharmaceutical Sciences, School of Pharmacy; ²Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261; and ³Department of Pharmacology, Center for Lung Biology, University of South Alabama, College of Medicine, Mobile, Alabama 36688

Submitted 8 December 2003 ; accepted in final form 11 May 2004

Although the CpG DNA immune response mediated by Toll-like receptor 9 (TLR9) has been extensively studied in a number of immune cells, the response to CpG DNA in endothelial cells (EC) is not well understood. In this study, we show that both mouse and rat lung EC display constitutive expression of TLR9 mRNA. Exposure to CpG DNA induced a potent proinflammatory response as manifested by an increased expression of IL-8 and ICAM-1 in mouse pulmonary EC. The proinflammatary response was sensitive to chloroquine, consistent with a role of endosomal contribution. A role for p38 MAPK and NF-B pathway was apparent as the response was sensitive to inhibitors of p38 MAPK and NF-B but was not affected by inhibitors of ERK1/2. A synergistic effect of CpG DNA and LPS on the inflammatory response is consistent with multiple TLR interaction in EC. This study suggests a possible role for CpG DNA-mediated EC immune response in the host defense system. It also has important implications in plasmid DNA-mediated pulmonary endothelium gene transfer.

CpG motif; lung

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