HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/1/L43    most recent 00096.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Chailley-Heu, B. Articles by Bourbon, J. R. Search for Related Content PubMed PubMed Citation Articles by Chailley-Heu, B. Articles by Bourbon, J. R.

FGF-18 is upregulated in the postnatal rat lung and enhances elastogenesis in myofibroblasts

Physiopathologie et Thérapeutique Respiratoires, Institut National de la Santé et de la Recherche Médicale U492, Faculté de Médecine, Université Paris XII, Créteil, France

Submitted 17 March 2004 ; accepted in final form 31 August 2004

The fibroblast growth factors (FGFs) are key players in fetal lung development, but little is known about their status in postnatal lung. Here, we investigated the expression pattern of FGF-18 transcripts through the perinatal period and evidenced a sevenfold increase after birth that paralleled changes in elastin expression. In vitro, recombinant human (rh)FGF-18 had a mitogenic activity on day 21 fetal rat lung fibroblasts and stimulated its own expression in the latter, whereas FGF-2 inhibited it. At 50 or 100 ng/ml, rhFGF-18 increased the expression of -smooth muscle actin (-SMA; 2.5-fold), a characteristic marker of myofibroblasts, of tropoelastin (6.5-fold), of lysyl oxidase (2-fold), and of fibulins 1 and 5 (8- and 2.2-fold) in confluent fibroblasts isolated from fetal day 21 lung; similar results were obtained with fibroblasts from day 3 postnatal lungs. Elastin protein expression was also slightly increased in fetal fibroblasts. Lung analysis on day 4 in rat pups that had received rhFGF-18 (3 µg) on days 0 and 1 showed a 1.7-fold increase of tropoelastin transcripts, whereas -SMA transcripts were unchanged. In contrast, rhFGF-2 markedly decreased expression of elastin in vitro and in vivo and of fibulin 5 in vitro. In addition, vitamin A, which is known to enhance alveolar development, elevated FGF-18 and elastin expressions in day 2 lungs, thus advancing the biological increase. We postulate that FGF-18 is involved in postnatal lung development through stimulating myofibroblast proliferation and differentiation.

lung development; elastin; lysyl oxidase; fibulin; -smooth muscle actin; fibroblast growth factor-18

This article has been cited by other articles:

				O. Boucherat, M.-L. Franco-Montoya, C. Thibault, R. Incitti, B. Chailley-Heu, C. Delacourt, and J. R. Bourbon Gene expression profiling in lung fibroblasts reveals new players in alveolarization Physiol Genomics, December 19, 2007; 32(1): 128 - 141. [Abstract] [Full Text] [PDF]

				O. Boucherat, A. Benachi, A.-M. Barlier-Mur, M.-L. Franco-Montoya, J. Martinovic, B. Thebaud, B. Chailley-Heu, and J. R. Bourbon Decreased Lung Fibroblast Growth Factor 18 and Elastin in Human Congenital Diaphragmatic Hernia and Animal Models Am. J. Respir. Crit. Care Med., May 15, 2007; 175(10): 1066 - 1077. [Abstract] [Full Text] [PDF]

				P.-P. Kuang, X.-H. Zhang, C. B. Rich, J. A. Foster, M. Subramanian, and R. H. Goldstein Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L944 - L952. [Abstract] [Full Text] [PDF]

				N. Shigemura, M. Okumura, S. Mizuno, Y. Imanishi, A. Matsuyama, H. Shiono, T. Nakamura, and Y. Sawa Lung Tissue Engineering Technique with Adipose Stromal Cells Improves Surgical Outcome for Pulmonary Emphysema Am. J. Respir. Crit. Care Med., December 1, 2006; 174(11): 1199 - 1205. [Abstract] [Full Text] [PDF]

				M. Yi, R. Belcastro, S. Shek, D. Luo, M. Post, and A. K. Tanswell Fibroblast Growth Factor-2 and Receptor-1{alpha}(IIIc) Regulate Postnatal Rat Lung Cell Apoptosis Am. J. Respir. Crit. Care Med., September 1, 2006; 174(5): 581 - 589. [Abstract] [Full Text] [PDF]

				E. Lopez, O. Boucherat, M.-L. Franco-Montoya, J. R. Bourbon, C. Delacourt, and P.-H. Jarreau Nitric Oxide Donor Restores Lung Growth Factor and Receptor Expression in Hyperoxia-Exposed Rat Pups Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 738 - 745. [Abstract] [Full Text] [PDF]