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Am J Physiol Lung Cell Mol Physiol 288: L285-L293, 2005. First published October 1, 2004; doi:10.1152/ajplung.00275.2004
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Abrogation of apoptosis through PDGF-BB-induced sulfated glycosaminoglycan synthesis and secretion

Nicholas J. Cartel1,3 and Martin Post1,2,3

1Program in Lung Biology, Research Institute, and Department of Pediatrics, The Hospital for Sick Children, Toronto; and Departments of 2Physiology and 3Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

Submitted 22 July 2004 ; accepted in final form 30 September 2004

Platelet-derived growth factor (PDGF)-BB-stimulated glycosaminoglycan (GAG) synthesis/secretion in fetal lung fibroblasts is dependent on sequential activation of the PDGF {beta}-receptor, phosphatidylinositol 3-kinase (PI3K), the serine/threonine kinase Akt-1,2, and the GTPase Rab3D. Because the Akt pathway has been implicated in cell survival mechanisms, we investigated whether the pathway regulating GAG synthesis/secretion was antiapoptotic. PDGF-BB treatment protected fetal lung fibroblasts against serum starvation-induced apoptosis, whereas wortmannin, an inhibitor of PI3K, abrogated this protective effect. Transfection of constitutively active Akt into fetal lung fibroblasts also safeguarded the cells from apoptosis induced by serum starvation. To determine whether the antiapoptotic response was due, at least in part, to GAGs, we treated lung fibroblasts with {beta}-D-xyloside as well as with topically applied GAGs, specifically those produced by fetal lung fibroblasts. {beta}-D-xyloside increased GAG synthesis/secretion and diminished apoptosis. Application of sulfated GAGs, chondroitin sulfate, and heparan sulfate, but not nonsulfated hyaluronan, also resulted in diminished apoptosis. Moreover, topically applied sulfated GAGs increased Bcl-associated death promoter phosphorylation and diminished caspase-3 and -7 cleavage, indicating an antiapototic response. These data are compatible with the PDGF-BB-GAG signaling pathway regulating programmed fibroblast death in the fetal lung.

platelet-derived growth factor


Address for reprint requests and other correspondence: M. Post, Program in Lung Biology, Research Institute, The Hospital for Sick Children, 555 Univ. Ave., Toronto, Ontario, Canada M5G 1X8 (E-mail: martin.post{at}sickkids.ca)




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Chondroitin-4-Sulphate Reduced Oxidative Injury in Caerulein-Induced Pancreatitis in Mice: The Involvement of NF-{kappa}B Translocation and Apoptosis Activation
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[Abstract] [Full Text] [PDF]


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