AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 288: L576-L584, 2005. First published November 24, 2004; doi:10.1152/ajplung.00380.2003
1040-0605/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/3/L576    most recent
00380.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eum, S.-Y.
Right arrow Articles by Martin, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eum, S.-Y.
Right arrow Articles by Martin, J. G.

IL-13 may mediate allergen-induced hyperresponsiveness independently of IL-5 or eotaxin by effects on airway smooth muscle

Seok-Yong Eum,1 Karim Maghni,2 Barbara Tolloczko,2 David H. Eidelman,2 and James G. Martin2

1Department of Microbiology, Yonsei University College of Medicine, Seoul, South Korea; and 2Meakins-Christie Laboratories, Department of Medicine, McGill University, Montreal, Canada

Submitted 5 November 2003 ; accepted in final form 22 November 2004

IL-13 is a mediator of allergen-induced airway hyperresponsiveness (AHR). The aim of this study was to evaluate whether eotaxin and IL-5 were implicated in the effects of IL-13 on allergen-induced AHR or whether IL-13 may exert its effects through direct actions on airway smooth muscle (ASM). To study this question airway inflammation and AHR were induced in mice by sensitization and subsequent challenge on three successive days with ovalbumin. A monoclonal anti-IL-13 antibody administered before each challenge significantly reduced AHR without affecting airway eosinophilia. No changes of mRNA in BAL and lung tissues or protein levels in BAL of IL-5 or eotaxin were found following anti-IL-13 treatment. Combined injection of monoclonal anti-IL-5 and antieotaxin antibodies before each antigen challenge blocked airway eosinophilia but failed to reduce AHR. IL-13 induced calcium transients in cultured murine ASM cells and augmented the calcium and contractile responses of these cells to leukotriene D4. These results suggest that IL-13 plays an important role in allergen-induced AHR and is important in the early phases of the inflammatory process. Its effects on AHR are mediated independently of IL-5 and eotaxin and may involve a direct effect on ASM to augment its responsiveness.

asthma; interleukin-13; airway eosinophilia; calcium signals; mice


Address for reprint requests and other correspondence: J. G. Martin, Meakins-Christie Laboratories, McGill Univ., 3626, rue St-Urbain, Montreal, Qc, Canada H2X 2P2 (E-mail: james.martin{at}mcgill.ca)




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
T. R. Johnson, M. E. Rothenberg, and B. S. Graham
Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein
J. Leukoc. Biol., September 1, 2008; 84(3): 748 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. Moynihan, B. Tolloczko, M.-C. Michoud, M. Tamaoka, P. Ferraro, and J. G. Martin
MAP kinases mediate interleukin-13 effects on calcium signaling in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L171 - L177.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
H. S. M. Farghaly, I. S. Blagbrough, D. A. Medina-Tato, and M. L. Watson
Interleukin 13 Increases Contractility of Murine Tracheal Smooth Muscle by a Phosphoinositide 3-kinase p110{delta}-Dependent Mechanism
Mol. Pharmacol., May 1, 2008; 73(5): 1530 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Parameswaran, K. Radford, A. Fanat, J. Stephen, C. Bonnans, B. D. Levy, L. J. Janssen, and P. G. Cox
Modulation of Human Airway Smooth Muscle Migration by Lipid Mediators and Th-2 Cytokines
Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 240 - 247.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. T. Kasaian, D. D. Donaldson, L. Tchistiakova, K. Marquette, X.-Y. Tan, A. Ahmed, B. A. Jacobson, A. Widom, T. A. Cook, X. Xu, et al.
Efficacy of IL-13 Neutralization in a Sheep Model of Experimental Asthma
Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 368 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Jaradat, C. Stapleton, S. L. Tilley, D. Dixon, C. J. Erikson, J. G. McCaskill, H. S. Kang, M. Angers, G. Liao, J. Collins, et al.
Modulatory Role for Retinoid-related Orphan Receptor {alpha} in Allergen-induced Lung Inflammation
Am. J. Respir. Crit. Care Med., December 15, 2006; 174(12): 1299 - 1309.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Matsubara, T. Koya, K. Takeda, A. Joetham, N. Miyahara, P. Pine, E. S. Masuda, C. H. Swasey, and E. W. Gelfand
Syk Activation in Dendritic Cells Is Essential for Airway Hyperresponsiveness and Inflammation
Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 426 - 433.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.