HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/6/L1171    most recent 00237.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Katagiri, Y. Articles by Goto, K. Search for Related Content PubMed PubMed Citation Articles by Katagiri, Y. Articles by Goto, K.

Expression and localization of diacylglycerol kinase isozymes and enzymatic features in rat lung

¹Department of Anatomy and Cell Biology and ²First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata; ³Department of Laboratory Medicine, Iwate Medical University School of Medicine, Morioka; ⁴Department of Cell Biology, Division of Histology, Tohoku University Graduate School of Medical Science, Sendai; and ⁵Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo, Japan

Submitted 25 June 2004 ; accepted in final form 17 February 2005

Diacylglycerol kinase (DGK) catalyzes phosphorylation of diacylglycerol to generate phosphatidic acid, and both molecules are known to serve as second messengers as well as important intermediates for the synthesis of various lipids. In this study, we investigated the spatiotemporal expression patterns of DGK isozymes together with the developmental changes of the mRNA expression and enzymatic property in rat lung. Northern blot and RT-PCR analyses showed that mRNAs for DGK, -, and - were detected in the lung. By immunohistochemical examination, DGK and - were shown to be coexpressed in alveolar type II cells and macrophages. Interestingly, these isozymes were localized at distinct subcellular locations, i.e., DGK in the cytoplasm and DGK in the nucleus, suggesting different roles for these isozymes. In the developing lung, the expression for DGK and - was transiently elevated on embryonic day 21 (E21) to levels approximately two- to threefold higher than on postnatal day 0 (P0). On the other hand, the expression for DGK was inversely elevated approximately twofold on P0 compared with that on E21. These unique changes in the expression pattern during the perinatal period suggest that each isozyme may play a distinct role in the adaptation of the lung to air or oxygen breathing at birth.

phosphoinositide; spatiotemporal expression patterns