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This Article Full Text Full Text (PDF) All Versions of this Article: 293/6/L1419    most recent 00418.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Takemoto, K. Articles by Ishiyama, H. Search for Related Content PubMed PubMed Citation Articles by Takemoto, K. Articles by Ishiyama, H.

Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma

¹Shin Nippon Biomedical Laboratories, Tokyo; ²Department of Public Health, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama; ³Third Institute of New Drug Discovery, Otsuka Pharmaceutical Company Limited, Tokushima; ⁴Department of Surgical Pathology, Yamaguchi University Hospital, Yamaguchi; Departments of ⁵Environmental and Preventive Medicine and ⁶Viral Infection and International Health, Graduate School of Medical Science, Kanazawa University, Kanazawa; and ⁷Division of Morphological Analysis, Faculty of Medicine, Department of Anatomy, Biology, and Medicine, Oita University, Oita City, Japan

Submitted 19 October 2006 ; accepted in final form 4 September 2007

Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 µg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0–4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.

NC/Nga; mite; reactive nitrogen species; airway remodeling

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