Reply

Joanne Engel, Michael Matthay

to the editor: We thank the correspondents for their thoughtful comments. We apologize for omitting the references to which they refer. Because our study was focused on the role of simple sugars, we did not comprehensively catalog all of the changes that have been reported in cells expressing Δ508CFTR. We would also like to emphasize that our mouse infection models were carried out in the context of a wild-type CFTR gene. We postulate that inhalation of simple sugars could be used to enhance the efficiency of conventional antibiotic therapy for acute or chronic pneumonia. Please note that in the discussion, we did specifically address the question of sugar multivalency as follows: “Our finding that a single sugar at a concentration equivalent to the mixture neither inhibited binding in vitro nor reduced bacterial lung burden or lung damage in vivo suggests that P. aeruginosa interacts with multiple sugar residues on glycan chains and that these targets need to be blocked simultaneously to attenuate bacterial binding.”