Lymphangioleiomyomatosis (LAM) is a rare neoplastic disease entailing cystic destruction of the lungs and progressive respiratory failure. LAM lungs are histologically characterized by the proliferation of smooth muscle-like cells (LAM cells) and an abundance of lymphatic vessels. To elucidate the pathophysiologic processes of LAM, cell type-specific analyses are required. However, no method exists for isolating the individual types of cells in LAM lesions. Therefore, we established a fluorescence-activated cell sorting (FACS)-based method for the direct isolation of LAM cells and other various cellular components from LAM-affected lung tissue. We obtained LAM-affected lung tissue from resections or transplant recipients and prepared single-cell suspensions. FACS, immunohistochemical, and molecular analysis were used cooperatively to isolate HMB45-positive LAM cells with TSC2 loss-of-heterozygosity (LOH). Using a combination of antibodies against an epithelial cell adhesion molecule (EpCAM) and podoplanin, we fractionated CD45-negative lung cells into three groups: lymphatic endothelial cells (LEC) (EpCAM-/podoplaninhi subset), alveolar Type II cells (EpCAMhi/podoplanin- subset), and mesenchymal cells (EpCAM-/podoplanin-/low subset). During subsequent analysis of HMB45 expression, as a LAM-specific marker, we clearly identified LAM cells in the mesenchymal cell population. We then discovered that CD90+/CD34- cells in the mesenchymal cell population are not only positive for HBM45 but also had TSC2 LOH. These isolated cells were viable and subsequently amenable to cell culture. This method enables us to isolate LAM cells and other cellular components including LAM-associated LEC from LAM-affected lung tissues, providing new research opportunities in this field.
- fluorescence-activated cell sorting
- loss of heterozygosity
- lymphangioleiomyomatosis cells
- lymphatic endothelial cells
- he tuberous sclerosis complex genes
- Copyright © 2015, American Journal of Physiology - Lung Cellular and Molecular Physiology