Table 1.

Effects of increasing concentrations of CSE on amiloride-inhibited and UTP- and forskolin-stimulated Isc in HBECs

BaselineCSEAmilUTP PeakUTP PlateauForsk PeakForsk PlateauBumetanide
Control (0 CSE)25±2.5025.2±2.8013.9±2.7930.9±3.8419.3±2.5134.8±2.6534.8±3.5223.8±3.61
5% CSE23.6±1.9319.6±1.343.8±0.4617.1±2.515.7±0.7523±2.1615.9±1.078.7±0.58
10% CSE20.8±1.1318.6±1.064.0±0.5915.2±2.806.5±0.8614.3±1.0710.8±1.048.8±1.16
20% CSE21.9±1.5018.6±1.476.6±1.2514.6±1.376.3±0.5710.7±0.818.5±0.807.5±0.87
40% CSE21±0.9619.7±0.848.4±1.3111.2±1.445.72±0.519.18±1.107.52±1.266.94±1.18
  • Values (in μA/cm2) are mean ± SE short-circuit current (Isc) for n = 8 (control), 6 [5% cigarette smoke extract (CSE)], 5 (10% CSE), 7 (20% CSE), or 5 (40% CSE). HBEC, human bronchial epithelial cell; Amil, amiloride; Forsk, forskolin.