Table 1.

ELISA results following mixing of SP-A1 (6A2) and SP-A2 (1A0) variants at different concentrations

Ratio of SP-A1: SP-A2Concentration of SP-A1, ng/100 μlConcentration of SP-A2, ng/100 μlSP-A1 Antibody Optical Density Readings
100:19910.36–0.37
10:190100.36–0.37
5:180200.36–0.37
1:150500.31
1:520800.25
1:1010900.19
1:1001990.10
  • Relative immunoreactivity with SP-A1 antibody is shown for SP-A1 and SP-A2 protein mixtures of different ratios at a final concentration of 100 ng each sample.